Sample ID	User ID	Date 	Time 	ng/ul 	A260 	A280 	260/280 	260/230 	Constant 	Cursor Pos.	Cursor abs.	340 raw
6G	Default	5/11/12	2:47 PM	1413.53	35.338	17.879	1.98	0.78	40	230	45.378	1.513
4M	Default	5/11/12	2:53 PM	1299.38	32.485	17.53	1.85	0.59	40	230	55.004	4.421
12M	Default	5/11/12	2:54 PM	341.04	8.526	4.411	1.93	0.8	40	230	10.647	1.167
20G	Default	5/11/12	2:55 PM	348.13	8.703	4.548	1.91	0.82	40	230	10.59	0.684
25M	Default	5/11/12	2:56 PM	385.01	9.625	5.037	1.91	0.79	40	230	12.225	0.561
29G	Default	5/11/12	2:57 PM	753.94	18.848	9.591	1.97	0.8	40	230	23.652	1.151
35G	Default	5/11/12	2:58 PM	390.34	9.758	5.04	1.94	0.94	40	230	10.399	0.376
41M	Default	5/11/12	2:59 PM	416.46	10.412	5.375	1.94	1.12	40	230	9.335	1.077




Calculations for cDNA												
Sample ID	ng/ul 	ng/17.75uL	ug/17.75uL	uL RNA for 1 ug	H2O to add (uL)	Primer added (uL)						
4M	1299.38	23064	23.06	0.77	16.98	0.5						
6G	1413.53	25090.16	25.09	0.71	17.04	0.5
12M	341.04	6053.46	6.05	2.93	14.82	0.5
20G	348.13	6179.31	6.18	2.87	14.88	0.5
25M	385.01	6833.93	6.83	2.6	15.15	0.5
29G	753.94	13382.44	13.38	1.33	16.42	0.5
35G	390.34	6928.54	6.93	2.56	15.19	0.5
41M	416.46	7392.17	7.39	2.4	15.35	0.5


Want 1ug of RNA						
Use 17.75uL RNA is max						



Calculations for Dnase						
Sample ID	ng/ul 	ug/ul	volume for 5ug	H2O to add		
4M	1299.38	1.29938	3.847989041	40.15201096
6G	1413.53	1.41353	3.537243638	40.46275636
12M	341.04	0.34104	14.66103683	29.33896317
20G	348.13	0.34813	14.36245081	29.63754919
25M	385.01	0.38501	12.98667567	31.01332433
29G	753.94	0.75394	6.631827466	37.36817253
35G	390.34	0.39034	12.8093457	31.1906543
41M	416.46	0.41646	12.00595495	31.99404505